RealCycler MENEHI enables the detection of Neisseria meningitidis, Streptococcus pneumoniae, and Haemophilus influenzae in clinical samples. These bacteria are major causes of bacterial meningitis and other severe infections such as septicemia. Neisseria meningitidis is known for causing outbreaks of meningitis, Streptococcus pneumoniae is a common cause of pneumonia and meningitis, and Haemophilus influenzae, particularly type b (Hib), can lead to meningitis, especially in young children. Early detection of these bacteria is crucial for initiating appropriate treatment and preventing potentially life-threatening complications.